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LIGATITAN the final step in the construction of a recombinant plasmid is connecting the insert dna gene or fragment of interest into a compatibly digested vector backbone this is accomplished by covalently connecting the sugar backbone of the two dna fragments this reaction called ligation is performed by the T4 dna ligase enzyme ligatitan LIGA188 adalah salah satu situs mix parlay bola dengan pasaran paling lengkap serta agen parlay bola paling di cari di indonesia ligatitan